The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:100 - 1:200
Western blot analysis of extracts of THP-1 cells, using PECAM1 antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using PECAM1 antibody.
Immunohistochemistry of paraffin-embedded human liver injury using PECAM1 antibody.
Immunohistochemistry of paraffin-embedded human kidney injury using PECAM1 antibody.
Background:
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.