A synthetic peptide of human Symmetric DiMethyl-Histone H4-R3
Calculated molecular weight
11kDa
Observed molecular weight
13kDa
Swiss Prot
P62805
Gene ID (NCBI)
8370
Alternative Names
FO108;H4;H4/n;H4F2;H4FN;HIST2H4
Application Notes:
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
IF
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H4-R3 antibody.
Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H4-R3 antibody.
Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H4-R3 antibody.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.