A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3
Calculated molecular weight
15kDa
Observed molecular weight
17kDa
Swiss Prot
P68431
Gene ID (NCBI)
8356
Alternative Names
H3/j;H3FJ
Application Notes:
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
IF
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R17 antibody.
Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R17 antibody.
Immunohistochemistry of paraffin-embedded human stomach using DiMethyl-Histone H3-R17 antibody.
Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R17 antibody.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.