- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-04427c
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Alpha-N-acetylgalactosaminidase,NAGA
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Alpha- N- acetylgalactosaminidase, NAGA ELISA KIT
ELI-04427c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Alpha- N- acetylgalactosaminidase, NAGA ELISA KIT is for the quantitative in vitro determination of Chicken NAGA concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Alpha- N- acetylgalactosaminidase, NAGA ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken NAGA in the sample, this Chicken Alpha- N- acetylgalactosaminidase, NAGA ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken NAGA concentration. The concentration of Chicken NAGA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验一篇文献中的ELISA方法如下：Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
CANDOR BIOSCIENCE: 关于ELISA板稳定性的技术探讨与比较
捕获分子的构象。尽管其优异的保护性能，但形成的层很容易溶解，因此稳定过的ELISA板储存后无需额外洗涤，样品可以直接添加入其中。 除了保持分析性能，现代固相稳定剂也有助于优化生产过程。更长的保质期允许更多的生产批次，因为试剂盒可以保存更长的时间。这不仅降低了成本，因为它节省了批量放行和机器设置时间，而且还减少了批间差异，因为客户可以延长使用同一批的时间。现代稳定剂（如Liquid Plate Sealer®）的高批次间一致性进一步补充了这一点。更长的保质期也有利于手工实验室分析，因为它们经常
结构不同，这决定了它们的抗原性也不同。IgG 和IgM 的重链分别称为γ(gamma )链和μ(mu )链。重链和轻链的 N 端的氨基酸排列顺序因各种抗体而异，称为可变区，分别用VH 和VL 表示。两者构成抗体的抗原结合部位，只与相应的抗原决定簇匹配，发生特异性结合，是抗体专一性结合抗原的结构基础。 IgG 可被木瓜蛋白酶分解为三个区段，其中两个相同的区段称抗原 结合片段(Fab )。每个Fab 都保存结合抗原的能力，但只有一个抗原结合位点，是单价的，与抗原结合后不出
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