- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- CELI-66053c
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Vitamin B12,VB12
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Vitamin B12, VB12 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Vitamin B12,cobalamin,vitamin B-12,vb12
Search name
Chicken Vitamin B12 ELISA KIT ,Chicken cobalamin ELISA KIT ,Chicken vitamin B-12 ELISA KIT ,Chicken vb12 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Chicken Vitamin B12 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin B12, During the reaction, Vitamin B12 in the sample or standard competes with a fixed amount of biotin-labeled Vitamin B12 for sites on a pre-coated Monoclonal antibody specific to Vitamin B12. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Vitamin B12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人维生素D ( Vitamin D )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Vitamin D 单抗包被于酶标板上，标准品和样品中的 Vitamin D与单抗结合，加入生物
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人维生素D ( Vitamin D )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Vitamin D 单抗包被于酶标板上，标准品和样品中的 Vitamin D与单抗结合，加入生物
Protein and Vitamin Production in Bacillus megaterium
are outlined. Practical examples for the directed development of B. megaterium to a vitamin B12 production host are also described.
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