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- 技术资料
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见产品外包装
- 英文名:
Permeabilization Wash Buffer
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
150ml
名称:Permeabilization Wash Buffer 破膜剂
CAS:N/A
产品说明:本产品常应用于细胞生物学研究等相关实验中.
产品详情:请咨询 <上海经科化学科技有限公司-客服>
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文献和实验Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
Harvest (10,000 rpm for 5 min) 24 h grown (in LB pH 7.5, 37 o C, 200 rpm) Escherichia coli , Enterobacter aerogenes or Pseudomonas aeruginosa cells. Wash once with 0.05 M potassium phosphate (KPi) bufferpH
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
to get through these as quickly as possible. 1. Spin down beads @10K, 1min, RT. 2. Aspirate supernatant. 3. Wash with 1ml of the following buffers and mix by inverting 3 or 4 times. Only aspirate supernatant to 0.1ml marking so no beads are lost: o FA lysis buffer
Fixation and permeabilization protocol 5/30/91 Mike Finney
from an unstarved plate with buffer. Wash them free of bacteria with water, leaving them in water in a microfuge tube on ice. - Add cold 2X MRWB to a final concentration of 1X and 20% formaldehyde to a final concentration of 1-4% (total volume usually 1 ml
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