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- 英文名:
Nuclear and Cytoplasmic Protein Extraction Kit
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
50T/100T
| 规格: | 50T | 产品价格: | ¥580.0 |
|---|---|---|---|
| 规格: | 100T | 产品价格: | ¥980.0 |
名称:Nuclear and Cytoplasmic Protein Extraction Kit 细胞核/细胞浆蛋白抽提试剂盒
CAS:N/A
产品说明:本产品常应用于蛋白研究等相关实验中.
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文献和实验PMSF (1 mM) Protease Inhibitor Cocktail, Sigma (Cat. no. P-2714) Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210) Or Cell Extraction Buffer Formulation 100 mM Tris, pH 7.4 2 mM Na3 VO4 100 mM NaCl
Preparation of nuclear extract and cytoplasmic extract
Solutions:Buffer A (Hypotonic Buffer): 1L10 mM HEPES pH 7.9 10 ml 1M HEPES,pH 7.91.5 mM MgCl2 1.5 ml 1M MgCl210 mM KCl3.33 ml 3M KCl0.5 mM DTT 500 µl 1M DTT0.2 mM PMSF 1 ml 0.2 M PMSFBuffer B (S100 extraction buffer): 500 ml0.3 M HEPES pH 7.9 150 ml
Detection of Cytoplasmic and Nuclear Functions of mTOR by Fractionation
rapamycin. These findings further demonstrate that the here presented and detailly described fractionation procedure is a valuable tool to study protein localization and cytoplasmic/nuclear protein shuttling in the context of expanding mTOR signalling.
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