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- 英文名:
NMY51 Chemically Competent Cell
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
10×100μl/50×100μl
| 规格: | 10×100μl | 产品价格: | ¥290.0 |
|---|---|---|---|
| 规格: | 50×100μl | 产品价格: | ¥1320.0 |
名称:NMY51 Chemically Competent Cell 酵母菌化学感受态细胞
CAS:N/A
产品说明:本产品常应用于分子生物学等相关实验中.
产品详情:请咨询 <上海经科化学科技有限公司-客服>
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文献和实验Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
Transforming chemically competent cells
Method Thaw TSS cells on ice. Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). Note: If you are adding small volumes (~1μl), be careful to mix the culture well. Diluting the plasmid
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
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