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- 英文名:
BL21 Star(DE3)Competent Cell
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
10×100μl/50×100μl
| 规格: | 10×100μl | 产品价格: | ¥396.0 |
|---|---|---|---|
| 规格: | 50×100μl | 产品价格: | ¥1696.0 |
名称:BL21 Star(DE3)Competent Cell 大肠杆菌化学感受态细胞
CAS:N/A
产品说明:本产品常应用于分子生物学等相关实验中.
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文献和实验CCMB buffer to make chemical competent cell
, but you need a big clone or several clones. :D LB media is OK, OD less than 0.8 is OK This protocol has been tested on TOP10, MachI and BL21(DE3) , DH5α cells. 1 Preparing glassware and media 2 Preparing seed stocks 3 Preparing competent cells 4 CCMB80
TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
(see table). Expression vector appropriate host strain pBAD vectors Top10, LMG194 pET vectors BL21 (DE3) pGEX vectors BL21 pMal vectors BL21, TB1 pProEx vectors BL21, DH10B pQE vectors M15 or M15 [pREP4] pRSET vectors BL21 (DE3) pLysS
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