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- 保存条件:
见产品外包装
- 英文名:
BCFL AM(Superior replacement for BCECF AM)pH
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
1mg
名称:BCFL AM(Superior replacement for BCECF AM)pH荧光探针
CAS:N/A
产品说明:本产品常应用于细胞生物学研究等相关实验中.
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文献和实验6xffis-Ni-NTA Chromatography as a Superior Technique in Recombinant Protein Expression/Purification
washed away under stringent conditions, yet the bound proteins can be gently eluted by competition with imidazole, or a slight reduction in pH. Moreover, because the interaction is independent of the tertiary structure of the tag, 6xHis labeled proteins
Recombineering/Lambda red-mediated gene replacement
Transformation Buffer 10 mM Pipes 15 mM CaCl2 250 mM KCl Titrate to pH 6.7 before adding MnCl2 55 mM MnCl2 Filter sterilize Incubate on ice for 10 min
Crystallization of Kinesin Family Motor Proteins
parameters.Crystals of monomeric rat Kinesin-1Table 1: Crystallization conditions for kinesin motor protein constructsConstructMethodConditionsReferencesHuman Kinesin-1 hK349Sitting drop 4°C5 mg/ml protein in 50 mM Na-acetate, pH 4.6, 75 mM KCl, 3.5% (w/v
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