Toluidine Blue O

Less common disorders:O

if presents within first few days of life. Recurring fractures Thin skin Blue sclerae Poor teeth Hypermobility of the joints Progressive deafness

Transformation of Electrocompetent E. coli with Blue/White selection

to a bacteria tube. Repeat for additional DNA samples and control DNA. Rotate at 37ºC x30 min. to allow the bacteria to recover. Meanwhile plate LB/Amp plates with IPTG + X-gal if blue/white selection of colonies will be performed

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder