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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见产品外包装
- 英文名:
TES,Free Acid Tris
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
7365-44-8
- 规格:
25g
名称:TES,Free Acid Tris乙磺酸
CAS:7365-44-8
产品说明:本产品属于生化试剂,常用于各种实验中..
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文献和实验How to make DEPC-treated water and Tris Buffer
first, and then dissolve Tris to make the appropriate buffer. Trace amounts of DEPC will modify purine residues in RNA by carboxymethylation. Carboxymethylated RNA is translated with very low efficiency in cell-free systems. However, its ability to form DNA:RNA or RNA:RNA
Acid Phenol Yeast RNA Preparation
ml conical tube. Centrifuge 4 min @ 4 deg. Remove supernatant. Quickly resuspend pellet in 800 microliters cold TES and freeze in dry ice or liquid N2.3. Warm acid phenol in H2O bath to 65 deg.4. Thaw cells briefly on ice.5. Add 800 microliters warm
cold water and transfer to 15 ml conical tube. Centrifuge 4 min @ 4 deg. Remove supernatant. Quickly resuspend pellet in 800 microliters cold TES and freeze in dry ice or liquid N2. 3. Warm acid phenol in H2O bath to 65 deg. 4. Thaw cells briefly
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