- ￥2260
- LSM Bio
- 进口
- PAab09799
- 2025年07月17日
- ELISA, IHC, WB
- E. coli - derived recombinant protein
- Human, Mouse, Rat
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
vinculin
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
polyclonal
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA, IHC, WB
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
vinculin
- 抗体英文名：
Vinculin antibody
- 抗体名：
Vinculin antibody
- 规格：
100μl
Vinculin antibody
Product Name Vinculin antibody
Catalog No PAab09799
Packing 100ul
Form liquid
Alternative Name CMD1W, Metavinculin, MVCL, VCL, Vinculin
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Immunogen vinculin
Specificity Human, Mouse, Rat
Tested Application ELISA, IHC, WB
Recommended dilution WB: 1:500-1:2000; IHC: 1:50-1:200
IHC use Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using PAab09799( Vinculin Antibody) at dilution of 1:100
WB use
HepG2 cells were subjected to SDS PAGE followed by western blot with PAab09799(Vinculin Antibody) at dilution of 1:1000
Protein Information
Gene ID 7414
Uniprot ID P18206
Calculated MW 124 kDa
Research Area Immunology, Cardiovascular
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- 作者
- 内容
- 询问日期
文献和实验. 7.TRITC,10% on celite (Research Organics). 8.Bio-Beads SM-2 in 0.7x15 column. Procedure (perform under reduced light) 1.Get 1-2 mg vinculin,stored in 2 mM Pipes,0.02% Azide,pH 7.6,in liquid nitrogen.Thaw quickly in warm water and chill in ice. 2.Mix
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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