- ￥2260
- LSM Bio
- 进口
- PAab09780
- 2025年07月06日
- ELISA, WB, IHC, FC
- E. coli - derived recombinant protein
- Human, Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
protein tyrosine phosphatase, receptor type, C
- 亚型：
IgG2a
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
monoclonal
- 标记物：
Non-conjugated
- 适应物种：
Human, Pig
- 保质期：
6个月
- 抗原来源：
Mouse
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA, WB, IHC, FC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
protein tyrosine phosphatase, receptor type, C
- 抗体英文名：
CD45 antibody
- 抗体名：
CD45 antibody
- 规格：
100μl
CD45 antibody
Product Name CD45 antibody
Catalog No PAab09780
Packing 100ul
Form liquid
Alternative Name B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200
Purification Protein A+G purification
Purity 95% as determined by SDS-PAGE
Host Mouse
Isotype IgG2a
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
CD45, also known as leukocyte common antigen(LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation.
Immunogen protein tyrosine phosphatase, receptor type, C
Specificity Human, Pig
Tested Application ELISA, WB, IHC, FC
Recommended dilution WB: 1:1000-1:10000; IHC: 1:20-1:200
IHC use Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using PAab09780(CD45 Antibody) at dilution of 1:300. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
WB use
Raji cells were subjected to SDS PAGE followed by western blot with PAab09780(CD45 Antibody) at dilution of 1:1000
Protein Information
Gene ID
Uniprot ID P08575
Calculated MW 180-220 kDa
Research Area Signal Transduction, Metabolism, Cardiovascular, Immunology, Cancer, Neuroscience, Stem Cells
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文献和实验CD45 由一类结构相似、分子量较大的跨膜蛋白组成，广泛存在于白细胞表面。CD45 分子在所有白细胞上都有表达，是细胞膜上信号传导的关键分子，在淋巴细胞的发育成熟，功能调节及信号传递中具有重要意义，其分布可作为某些 T 细胞亚群的分类标志。 图一：CD45/SSC-H与FSC-H/SSC-H分群对比 ❖ 如左图所示，通过 CD45 与 SSC-H，可以非常快速地圈出淋巴细胞亚群。 ❖ 如右图所示，使用 FSC-H 与 SSC-H 确定细胞群分布时，如果不染 CD45，加上有时红细胞
CD45/CD45RA/CD45RB/CD45RC/CD45RO
CD45/CD45RA/CD45RB/CD45RC/CD45RO CD45 常用单克隆抗体或代号： T29/33,BMAC1;(T200,B220) 主要表达细胞： Leu [NL] 分子质量(kDa)和结构： gp180- 240,白细胞共同抗原(LCA) 功 能： PTP酶,调节信号传导 CD45RA 常用单克隆抗体或代号： G1- 15,F8- 11- 13,Leu18,2H4;(限制性LCA) 主要表达细胞： Tsub,B,G,M
Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes
CD45 is a leukocyte specific protein tyrosine phosphatase and is a major lymphocyte cell surface glycoprotein (see refs. 1 –3 for reviews). CD45 has been shown to be important for the transmission of signals by the T and B cell antigen
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