The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
IF
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using AK1 antibody.
Immunohistochemistry of paraffin-embedded rat lung using AK1 Antibody.
Immunohistochemistry of paraffin-embedded rat kidney using AK1 Antibody.
Immunohistochemistry of paraffin-embedded human colon using AK1 Antibody.
Immunohistochemistry of paraffin-embedded human colon carcinoma using AK1 Antibody.
Immunohistochemistry of paraffin-embedded human esophagus using AK1 Antibody.
Immunohistochemistry of paraffin-embedded mouse heart using AK1 Antibody.
Immunofluorescence analysis of HeLa cells using AK1 antibody.
Background:
This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.