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- 详细信息
- 文献和实验
- 技术资料
- 库存:
货源充足
- 供应商:
LSM Bio
- 检测范围:
0.24ng/mL
- 检测方法:
夹心法/竞争法
- 应用:
Rat Tmem39a ELISA KIT allows for the in vitro quantitative determination of Tmem39a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
- 适应物种:
大鼠
- 标记物:
详见说明书
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 规格:
96T
Rat Tmem39a ELISA KIT
Product Name:Rat Tmem39a ELISA KIT
Packing:96T
Catalog No.:ELI-51921r
Gene Name:Tmem39a
Detect Range:0.78-50 ng/mL
Sensitivity:0.24ng/mL
Target Protein Name:Tmem39a
Alternative Name:Tmem39a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat Tmem39a ELISA KIT allows for the in vitro quantitative determination of Tmem39a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human LRP2 ELISA Kit has been pre-coated with an LRP2 antibody specific to Human Low-density lipoprotein receptor-related protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Low-density lipoprotein receptor-related protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Low-density lipoprotein receptor-related protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Low-density lipoprotein receptor-related protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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体的形成及下游信号通路的激活。表明 PI4KB 通过在 STING 周围合成 PI4P 促进 STING 的激活。有趣的是,SAC1 的敲低会引起的细胞内 PI4P 水平的异常升高,进而导致 STING 不依赖于 cGAS 的自激活。TMEM39A 是一个自身免疫性疾病相关基因且被报道参与细胞内的 PI4P 水平的调控 4。同样,TMEM39A 的敲低也会导致细胞内 PI4P 的水平的异常升高及 STING 的自激活,该发现有助于理解 TMEM39A 相关自身免疫疾病的致病机制。此外,SCAP
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
inhibitors (Sigma Cat. no. P-8340). Chill this solution using wet ice. 6. Homogenize at low speed for ~20 seconds. Make sure to keep cool and keep samples on wet ice. 7. Transfer samples into 1.7 ml microcentrifuge tubes. 8. Centrifuge at 14,000 x g
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