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Tmem39a/ Rat Tmem39a ELISA试剂盒

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  • ¥3280
  • LSM BIO
  • 0.24ng/mL
  • 详询
  • ELI-51921r
  • 2025年07月11日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      货源充足

    • 供应商

      LSM Bio

    • 检测范围

      0.24ng/mL

    • 检测方法

      夹心法/竞争法

    • 应用

      Rat Tmem39a ELISA KIT allows for the in vitro quantitative determination of Tmem39a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

    • 适应物种

      大鼠

    • 标记物

      详见说明书

    • 样本

      大鼠血清、血浆或其它相关生物液体中天然和重组蛋白

    • 规格

      96T

    Rat Tmem39a ELISA KIT

    Product Name:Rat Tmem39a ELISA KIT
    Packing:96T

    Catalog No.:ELI-51921r

    Gene Name:Tmem39a

    Detect Range:0.78-50 ng/mL

    Sensitivity:0.24ng/mL

    Target Protein Name:Tmem39a

    Alternative Name:Tmem39a
    Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

    ELISA type:Sandwich ELISA Kit
    Product Description:Rat Tmem39a ELISA KIT allows for the in vitro quantitative determination of Tmem39a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

    ELISA Test Principle:
    The microtiter plate provided in Human LRP2 ELISA Kit has been pre-coated with an LRP2 antibody specific to Human Low-density lipoprotein receptor-related protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Low-density lipoprotein receptor-related protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Low-density lipoprotein receptor-related protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Low-density lipoprotein receptor-related protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

     

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    • Rat Liver Preparation

      inhibitors (Sigma Cat. no. P-8340). Chill this solution using wet ice. 6. Homogenize at low speed for ~20 seconds. Make sure to keep cool and keep samples on wet ice. 7. Transfer samples into 1.7 ml microcentrifuge tubes. 8. Centrifuge at 14,000 x g

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