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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat SERT
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat SERT ELISA Kit
Product Name:Rat SERT ELISA Kit
Packing:96T
Catalog No.:ELA-E2290r
Gene Name:SERT
Detect Range:0.156-10 ng/mL
Sensitivity:0.1ng/mL
Target Protein Name:SERT
Alternative Name:SERT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat SERT ELISA Kit allows for the in vitro quantitative determination of SERT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human LRP2 ELISA Kit has been pre-coated with an LRP2 antibody specific to Human Low-density lipoprotein receptor-related protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Low-density lipoprotein receptor-related protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Low-density lipoprotein receptor-related protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Low-density lipoprotein receptor-related protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Western Blot、ELISA等实验是目前实验室最常用的检测蛋白量的实验方法。然而许多研究人员都会在这些实验过程中遇到背景过高或信号太弱等问题,影响了结果分析和实验进度。如何更好地解决实验中遇到的这些问题?许多研究者花费了大量的时间和经费优化实验条件以求得到更好的结果,结果却往往差强人意。 为了更好地解决这个虽然很小,却困扰很大的问题--低信号/高背景,德国默克公司经验丰富的研发团队经过不断摸索开发出了SignalBoostTM 免疫信号增强试剂盒(货号:407207)。该试剂盒是一种
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