- ￥3280
- LSM BIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E1336r
- 2025年10月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat BrdU
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat BrdU ELISA Kit
Product Name:Rat BrdU ELISA Kit
Packing:96T
Catalog No.:ELA-E1336r
Gene Name:BrdU
Detect Range:1.56-100 U/L
Sensitivity:0.78U/L
Target Protein Name:BrdU
Alternative Name:BrdU
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat BrdU ELISA Kit allows for the in vitro quantitative determination of BrdU concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human LRP2 ELISA Kit has been pre-coated with an LRP2 antibody specific to Human Low-density lipoprotein receptor-related protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Low-density lipoprotein receptor-related protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Low-density lipoprotein receptor-related protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Low-density lipoprotein receptor-related protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Anti-BrdU-FITC (Becton Dickinson or Phoenix Flow) 0.15 M NaCl, 1.5 M NaCl 10 Paraformaldehype (kept as stock in -80°C) Tween 1M MgCl2 FACS Tubes Protocol: Cells in 96 well FACS plate Block with 24G-2 Surface stain cells
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 溴脱氧核苷尿嘧啶 ( BrdU )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 BrdU 单抗包被于酶标板上，标准品和样品中的 BrdU与单抗结合，加入生物素化的抗人 BrdU ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin
erik 哪地方有卖的？想买点试试！ lin123heng 我已经试用过EDU试剂了，做的是细胞爬片。因为流式要激发光550nm，我们实验室只有488nm。我用的是国产试剂，说明书写的一塌糊涂，最后我不得不自己完全改一个protocol.结果并不是很理想，因为我们照相机红色荧光不是很敏感，图片，没有上面的那么好。如果要我再选一次，我宁愿用PI或者是Brdu做流式。我买的试剂盒是1200元/20次。六孔板做的。 希望有兴趣的战友们实验前先看一看自己实验
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