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- Sino Biological
- 70014-DNAE
- 2025年08月12日
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50 µg
蛋白名称：IL2蛋白, IL2 protein
蛋白构建：A DNA sequence encoding the mature form of canine IL2 (Q29416) (Ala 21-Thr 155, 147Cys/Ser) was expressed, with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant Canine IL2 (cat:70014-DNAE) at 10 μg/mL can bind recombinant human IL2Ra-Fc (Cat:10165-H02H) with a linear range of 0.3125-20 μg/ml.
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant canine IL2 consists of 136 amino acids and has a calculated molecular mass of 15.6 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 15 kDa band.
蛋白NP号：Q29416
蛋白氨基酸序列：Ala21-Thr155,147Cys/Ser
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Antibody-IL2 Fusion Proteins for Tumor Targeting
as part of an antibody-cytokine fusion protein to the tumor tissue by a single chain fragment of variable regions (scFv) antibody recognizing a tumor-associated antigen. IL2 can moreover be combined with IL12 in a dual cytokine fusion protein, which simultaneously
) and recombination activating 1 or 2 gene (Rag1 or Rag2 ) knockout mice bearing a targeted mutation in the gene encoding the IL2 receptor gamma chain (IL2rγ ) have been reported. The addition of the mutated IL2rγ gene onto an immunodeficient mouse stock facilitates
基础。 传统的NK细胞扩增技术是在NK细胞培养体系中添加IL2、IL15等细胞因子以促进NK细胞增殖，但由于扩增效率不高且纯度不够未能得到广泛应用。近年来，有人采用基因工程的方法在K562细胞上同时表达IL-15、CD137L 等分子, 并以此细胞来刺激NK细胞的增殖。IL-15和CD137L联合刺激是PBMC 中NK细胞特异性扩增的基础：作为膜蛋白，通过细胞间的接触刺激，用以诱导NK细胞扩增。IL15能够促进NK细胞的分化成熟，而IL21能够联合IL15分子促进NK细胞的分化成熟，所以本研究在稳定
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