人 IL-12蛋白|IL-12 protein|IL-12(Human, C-His)(产品说明)
蛋白名称:IL-12蛋白, IL-12 protein
蛋白构建:A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. The purified protein was biotinylated in vitro.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Arg 23 & Ile 23
蛋白分子量:The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa.
蛋白NP号:P29459.2 & NP_002178.2
蛋白氨基酸序列:Met1-Ser219 & Met1-Ser328
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.