人 IL-6蛋白|IL-6 protein|IL-6(Human, Native)(产品说明)
蛋白名称:IL-6蛋白, IL-6 protein
蛋白构建:A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus.
表达宿主:E. coli
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. 2.Immobilized human IL6 (Cat:GMP-10395-HNAE) at 10μg/mL (100μL/well) can bind human IL6R-His (Cat:10398-H08H) , the EC50 of human IL6R-His is 0.1-0.5 μg/mL.3. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.2-0.8 ng/mL. The specific activity of recombinant human IL-6 is approximately 3.2 x 105 IU/μg, which is calibrated against human IL-6 WHO International Standard (NIBSC code: 89/548).
蛋白内毒素:< 0.01 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human IL6 consists of 184 amino acids and has a calculated molecular mass of 20.9 kDa.
蛋白NP号:NP_000591.1
蛋白氨基酸序列:Val30-Met212
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles