Rat Heparin cofactor II ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Rat heparin cofactor II ELISA KIT ,Rat Heparin cofactor 2 ELISA KIT ,Rat SERPIND1 ELISA KIT ,Rat HCF2 ELISA KIT ,Rat HCF-2 ELISA KIT ,Rat C2 factor ELISA KIT ,Rat host cell factor C2 ELISA KIT ,Rat Serpin D1 ELISA KIT ,Rat HC-II;Protease inhibitor leuserpin-2 ELISA KIT ,Rat D22S673 ELISA KIT ,Rat HC2 ELISA KIT ,Rat HCF2 ELISA KIT ,Rat HCII ELISA KIT ,Rat HLS2 ELISA KIT ,Rat LS2 ELISA KIT ,Rat THPH10 ELISA KIT ,Rat leuserpin 2 ELISA KIT ,Rat protease inhibitor leuserpin-2 ELISA KIT ,Rat serine proteinase inhibitor clade Dmember 1 ELISA KIT ,Rat serpin D1 ELISA KIT ,Rat serpin peptidase inhibitor clade D member 1 ELISA KIT ,Rat serpin family D member 1 ELISA KIT
Intended use
Rat Heparin cofactor II ELISA Kit allows for the in vitro quantitative determination of rat Heparin cofactor II concentrations in serum, Plasma, Urine,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat Heparin cofactor II ELISA Kit has been pre-coated with an antibody specific to Heparin cofactor II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Heparin cofactor II and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heparin cofactor II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Heparin cofactor II in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat Heparin cofactor II manual request
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