- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0422r
- 2025年07月05日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat myelin-associated glycoprotein,MAG
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat myelin- associated glycoprotein, MAG ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MAG,myelin associated glycoprotein,S-MAG; GMA; SIGLEC-4A; SIGLEC4A; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A, Myelin-associated glycoprotein,
Search name
Rat MAG ELISA KIT ,Rat myelin associated glycoprotein ELISA KIT ,Rat S-MAG ELISA KIT ,Rat GMA ELISA KIT ,Rat SIGLEC-4A ELISA KIT ,Rat SIGLEC4A ELISA KIT ,Rat sialic acid binding Ig-like lectin 4A ELISA KIT ,Rat sialic acid-binding immunoglobulin-like lectin 4A ELISA KIT ,Rat Myelin-associated glycoprotein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Myelin-associated glycoprotein concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin-associated glycoprotein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Myelin-associated glycoprotein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myelin-associated glycoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Myelin-associated glycoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Expression and Secretion of a Soluble Form of Myelin-Associated Glycoprotein (MAG)
Myelin-associated glycoprotein (MAG) was first identified as the major glycoprotein in the central nervous system (CNS) through 3 Hfucose-labeling experiments (1 ). It is expressed on the surface of glial cells of both the CNS and peripheral
Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium
, in its plasma-lemma. The oligodendroblast differentiates into the mitotically quiescent oligodendrocyte, a cell characterized by the presence of galactocerebroside in its plasmalemma. These oligodendrocytes also express other myelin-associated proteins
In vitro blood–brain barrier (BBB) models using primary cultured brain endothelial cells are important for establishing cellular and molecular mechanisms of BBB function. Co-culturing with BBB-associated cells especially
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