- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-48619r
- 2025年11月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit b
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat GNB3 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat GNB3 ELISA KIT ,Rat CSNB1H ELISA KIT ,Rat guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 ELISA KIT ,Rat G protein beta-3 subunit ELISA KIT ,Rat GTP-binding regulatory protein beta-3 chain ELISA KIT ,Rat guanine nucleotide binding protein (G protein) beta polypeptide 3 ELISA KIT ,Rat guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3 ELISA KIT ,Rat hypertension associated protein ELISA KIT ,Rat transducin beta chain 3 ELISA KIT ,Rat G protein subunit beta 3 ELISA KIT
Intended use
Rat GNB3 ELISA kit is for the in vitro quantitative determination of Rat GNB3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Rat GNB3 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GNB3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GNB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GNB3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat GNB3 ELISA kit Range 0.15-10ng/ml
GNB3 Related Products
> GNB3 ELISA KITS
Canine Guanine nucleotide- binding protein G(I)/G(S)/G(T) subunit beta- 3, GNB3 ELISA KIT
Human Guanine nucleotide- binding protein G(I)/G(S)/G(T) subunit beta- 3, GNB3 ELISA KIT
Mouse Guanine nucleotide- binding protein G(I)/G(S)/G(T) subunit beta- 3, Gnb3 ELISA KIT
Rat Guanine nucleotide- binding protein G(I)/G(S)/G(T) subunit beta- 3, Gnb3 ELISA KIT
> GNB3 Recombinant Protein
Human GNB3 Recombinant Protein
> GNB3 Antidodies
Anti GNB3 Antidody
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
On activation, G-protein-coupled receptors (GPCRs) exert many of their cellular actions through promotion of guanine nucleotide exchange on the Gα-subunit of heterotrimeric G-proteins to release free Gα-GTP and βγ-subunits. In membrane
[35S]GTPS Binding as an Index of Total G-Protein and G-Subtype-Specific Activation by GPCRs
binding step, Gα-subunit-specific antibodies can be used to investigate the Gα-protein subpopulations activated by receptors by immunoprecipitation of [35 S]GTPγS–Gα complexes and quantification by scintillation counting. Here, we describe a total [35 S
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