Rat Protein fem- 1 homolog A,

Rat Protein fem- 1 homolog A, FEM1a ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Protein fem-1 homolog A,FEM1a, FEM1aa,FEM1-alpha,Prostaglandin E receptor 4-associated protein, FEM1A,EPRAP; FEM1-alpha; prostaglandin E receptor 4-associated protein; protein fem-1 homolog A; fem-1 homolog a C. elegans

Search name

Rat Protein fem-1 homolog A ELISA KIT ,Rat FEM1a ELISA KIT ,Rat FEM1aa ELISA KIT ,Rat FEM1-alpha ELISA KIT ,Rat Prostaglandin E receptor 4-associated protein ELISA KIT ,Rat  FEM1A ELISA KIT ,Rat EPRAP ELISA KIT ,Rat FEM1-alpha ELISA KIT ,Rat prostaglandin E receptor 4-associated protein ELISA KIT ,Rat protein fem-1 homolog A ELISA KIT ,Rat fem-1 homolog a C elegans ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat protein fem-1 homolog a,FEM1a concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FEM1a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FEM1a and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FEM1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FEM1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.