- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-48470r
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Golgi apparatus protein 1,Glg1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Golgi apparatus protein 1, GLG1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GLG1,CFR-1; ESL-1; MG-160; MG160; E-selectin ligand 1; cysteine-rich fibroblast growth factor receptor; golgi sialoglycoprotein MG-160; golgi glycoprotein 1, Golgi apparatus protein 1
Search name
Rat GLG1 ELISA KIT , Rat GLG ELISA KIT,Rat CFR-1 ELISA KIT ,Rat ESL-1 ELISA KIT ,Rat MG-160 ELISA KIT ,Rat MG160 ELISA KIT ,Rat E-selectin ligand 1 ELISA KIT ,Rat cysteine-rich fibroblast growth factor receptor ELISA KIT ,Rat golgi sialoglycoprotein MG-160 ELISA KIT ,Rat golgi glycoprotein 1 ELISA KIT ,Rat Golgi apparatus protein 1 ELISA KIT, Rat Golgi apparatus protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Golgi apparatus protein 1 concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgi apparatus protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forGolgi apparatus protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Golgi apparatus protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Golgi apparatus protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Use of Polarized PC12 Cells to Monitor Protein Localization in the Early Biosynthetic Pathway
of these cells involves the expansion of the intermediate compartment (IC) between the rough endoplasmic reticulum (RER) and the Golgi apparatus. Tubules emerging from the vacuolar parts of the IC move to the developing neurites accumulating in their growth cones
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
are produced as soluble recombinant proteins by transiently transfecting a mammalian cell line. The proteins are purified using an oligo‐His tag using a bespoke purification apparatus (the “Protein Press”) and subsequently printed onto glass slides
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
apparatus. Aspirate with pasteur pipets as before. A triple wash is required, this time using the Nunc washer to fill and aspirate each row 3x simultaneously before moving on to the next row. Dry the wells by removing the remaining liquid with pasteur pipets
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