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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Sodium channel protein type 1 subunit alpha,Scn1a
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Scn1a ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
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Intended use
Rat Scn1a ELISA kit allows for the in vitro quantitative determination of Rat Scn1a concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Rat Scn1a ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Scn1a and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Scn1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Scn1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Order Scn1a ELISA kit by
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文献和实验Characterization of Calcium Channel Binding
.T., Naylor, M.J., Maybaum, T.A., Pearce, W.G., Koop, B., Fishman, G.A., Mets, M., Musarella, M.A., and Boycott, K.M. 1998. Loss‐of‐function mutations in a calcium‐channel alpha1‐subunit gene in Xp11.23 cause incomplete X‐linked congenital stationary
,与癫痫发生关系密切。 3. 电压阀门钠通道蛋白Ⅰ型 α 亚单位(sodium channel voltage-gated type I alpha,SCN1A)基因SCN1A 主要表达在中枢神经系统,其基因突变与全面性癫痫伴热性惊厥、Dravet 综合症、共济失调等神经系统疾病有关。SCN1A 基因的位点、类型的突变都可能会引起钠离子通道功能的失衡,使离子通道兴奋性失调,从而导致各种不同类型癫痫发生。 癫痫生物标记物能早期对癫痫做出预警作用,有效避免了将某些疾病误诊为癫痫给患者带来不必要的严重
ELISA Procedure for Measuring Serum Antibody Titer
assays are the most appropriate for measuring the titer of the antisera you have generated. ELISA Procedure for Measuring Serum Antibody Titer In this type of ELISA (Enzyme-Linked Immunosorbant Assay), the antigen (peptide or protein) is bound
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