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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Sex-determining region Y protein,Sry
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Sex determining region Y protein, SRY ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Sex-determining region Y protein,Testis-determining factor, SRY,SRXX1; SRXY1; TDF; TDY; essential protein for sex determination in Rat males; sex-determining region Y protein; sex-determining region on Y; testis-determining factor on Y; sex determining region Y
Search name
Rat Sex-determining region Y protein ELISA KIT ,Rat Testis-determining factor ELISA KIT ,Rat SRY ELISA KIT ,Rat SRXX1 ELISA KIT ,Rat SRXY1 ELISA KIT ,Rat TDF ELISA KIT ,Rat TDY ELISA KIT ,Rat essential protein for sex determination in Rat males ELISA KIT ,Rat sex-determining region Y protein ELISA KIT ,Rat sex-determining region on Y ELISA KIT ,Rat testis-determining factor on Y ELISA KIT ,Rat sex determining region Y ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat sex-determining region y protein,SRY concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SRY. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SRY and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SRY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SRY in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验当 Y 染色体消失,男性却依然存在!PNAS:科学家发现了性别决定的最新奥秘
道大学的研究团队揭示了一种独立于 Y 染色体的新的性别决定机制。研究人员在奄美刺鼠中发现了一段仅存在于雄性个体中的 DNA 重复序列,该重复序列位于 3 号染色体 Sox9 基因序列的上游,能在缺少 Sry 基因的情况下促进 Sox9 基因的表达,诱导睾丸形成。 这一结果表明奄美刺鼠的性别决定机制已经转移到常染色体——3 号染色体,这也是哺乳动物性别决定机制易位的首个例子。 该研究结果以 Turnover of mammal sex chromosomes in the Sry
Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA
An IgG-type antibody is a Y-shaped protein whose arms form two identical antigen-binding sites that are highly variable between different molecules. The stem of the Y is part of the constant region of an antibody and has very limited diversity
Determining In Vivo Phosphorylation Sites Using Mass Spectrometry
is the most studied protein post?translational modification (PTM) in biological systems, since it controls cell growth, proliferation, survival, and other processes. High?resolution/high mass accuracy mass spectrometers are used to identify protein phosphorylation
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