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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Parathymosin,Ptms
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Parathymosin, PTMS ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ParaT,PTMS,parathymosin,Zinc-binding 11.5 kDa protein,Znbp
Search name
Rat ParaT ELISA KIT ,Rat PTMS ELISA KIT ,Rat parathymosin ELISA KIT ,Rat Zinc-binding 11.5 kDa protein ELISA KIT ,Rat Znbp ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Parathymosin, PTMS concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parathymosin, During the reaction, Parathymosin in the sample or standard competes with a fixed amount of biotin-labeled Parathymosin for sites on a pre-coated Monoclonal antibody specific to Parathymosin. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Parathymosin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验类:· Serine proteases· Cysteine proteases· Aspartic acid proteases· Zinc metalloproteases图示:比色蛋白酶试验响应曲线。采用 Thermo Scientific Pierce 比色法蛋白酶检测试剂盒,通过与提供的胰蛋白酶标准品进行比较,测定 V-8 蛋白酶和颌下腺蛋白酶消化酪蛋白底物的活性。以上即为PTMs的概述(资料来自Thermo Fisher官网),欢迎留言评论
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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