Rat Hematopoietic prostaglandin D synthase, HPGDS ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Hematopoietic prostaglandin D synthase,H-PGDS,GST, GST TAG,Prostaglandin-H2 D-isomerase,Glutathione-requiring prostaglandin D synthase,Glutathione-dependent PGD synthase,GST class-sigma,Glutathione S-transferase HpgdsH-PGDS; Ptgds2; GST class-sigma; glutathione S-transferase; glutathione-dependent PGD synthase; glutathione-dependent PGD synthetase; glutathione-requiring prostaglandin D synthase; prostaglandin D2 synthase 2, hematopoietic; prostaglandin-H2 D-isomerase; hematopoietic prostaglandin D synthase
Search name
Rat Hematopoietic prostaglandin D synthase ELISA KIT ,Rat H-PGDS ELISA KIT ,Rat GST ELISA KIT ,Rat GST TAG ELISA KIT ,Rat Prostaglandin-H2 D-isomerase ELISA KIT ,Rat Glutathione-requiring prostaglandin D synthase ELISA KIT ,Rat Glutathione-dependent PGD synthase ELISA KIT ,Rat GST class-sigma ELISA KIT ,Rat Glutathione S-transferase HpgdsH-PGDS ELISA KIT ,Rat Ptgds2 ELISA KIT ,Rat GST class-sigma ELISA KIT ,Rat glutathione S-transferase ELISA KIT ,Rat glutathione-dependent PGD synthase ELISA KIT ,Rat glutathione-dependent PGD synthetase ELISA KIT ,Rat glutathione-requiring prostaglandin D synthase ELISA KIT ,Rat prostaglandin D2 synthase 2 ELISA KIT ,Rat hematopoietic ELISA KIT ,Rat prostaglandin-H2 D-isomerase ELISA KIT ,Rat hematopoietic prostaglandin D synthase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat hematopoietic prostaglandin d synthase,H-PGDS concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to H-PGDS, During the reaction, H-PGDS in the sample or standard competes with a fixed amount of biotin-labeled H-PGDS for sites on a pre-coated Monoclonal antibody specific to H-PGDS. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of H-PGDS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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