Rat H/ACA ribonucleoprotein complex subunit 1, GAR1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GAR1,GAR1 ribonucleoprotein; snoRNP protein GAR1; GAR1 ribonucleoprotein homolog; NOLA1; nucleolar protein family A member 1; nucleolar protein family A member 1,H/ACA small nucleolar RNPs, H/ACA ribonucleoprotein complex subunit 1,Nucleolar protein family A member 1,snoRNP protein GAR1,
Search name
Rat GAR1 ELISA KIT ,Rat GAR1 ribonucleoprotein ELISA KIT ,Rat snoRNP protein GAR1 ELISA KIT ,Rat GAR1 ribonucleoprotein homolog ELISA KIT ,Rat NOLA1 ELISA KIT ,Rat nucleolar protein family A member 1 ELISA KIT ,Rat nucleolar protein family A member 1 ELISA KIT ,Rat H/ACA small nucleolar RNPs ELISA KIT ,Rat H/ACA ribonucleoprotein complex subunit 1 ELISA KIT ,Rat Nucleolar protein family A member 1 ELISA KIT ,Rat snoRNP protein GAR1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat H/ACA ribonucleoprotein complex subunit 1concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toH/ACA ribonucleoprotein complex subunit 1, During the reaction,H/ACA ribonucleoprotein complex subunit 1in the sample or standard competes with a fixed amount of biotin-labeledH/ACA ribonucleoprotein complex subunit 1 for sites on a pre-coated Monoclonal antibody specific to H/ACA ribonucleoprotein complex subunit 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of H/ACA ribonucleoprotein complex subunit 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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