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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3,
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Alpha- ketoglutarate- dependent dioxygenase alkB homolog 3, ALKBH3 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ALKBH3,ABH3; DEPC-1; DEPC1; PCA1; alkylated DNA repair protein alkB homolog 3; alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; prostate cancer antigen 1; prostate cancer antigen-1; alkB, alkylation repair homolog 3 (E. coli)
Search name
Rat ALKBH3 ELISA KIT ,Rat ABH3 ELISA KIT ,Rat DEPC-1 ELISA KIT ,Rat DEPC1 ELISA KIT ,Rat PCA1 ELISA KIT ,Rat alkylated DNA repair protein alkB homolog 3 ELISA KIT ,Rat alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 ELISA KIT ,Rat prostate cancer antigen 1 ELISA KIT ,Rat prostate cancer antigen-1 ELISA KIT ,Rat alkB ELISA KIT ,Rat alkylation repair homolog 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Alpha- ketoglutarate- dependent dioxygenase alkB homolog 3, ALKBH3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALKBH3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ALKBH3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ALKBH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ALKBH3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Introduction of human 14-3-3 proteins
Biophysics,Medical College of Virginia,Virginia Commonwealth University,Richmond 23298-0614,USABiochem Biophys Res Commun 224: 103-7 (1996)CMP-NeuAc:GM1 alpha 2,3-sialyltransferase (ST-IV)was purified to homogeneity from rat brain.Microsequencing
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