- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-23698r
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Melanocortin receptor 4,Mc4r
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Melanocortin receptor 4,MC4R ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Melanocortin receptor 4, MC4R,melanocortin 4 receptor,MC4-R
Search name
Rat Melanocortin receptor 4 ELISA KIT ,Rat MC4R ELISA KIT ,Rat melanocortin 4 receptor ELISA KIT ,Rat MC4-R ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat melanocortin receptor 4,MC4-R concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MC4-R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MC4-R and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MC4-R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MC4-R in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Characterization of Melanocortin Receptors
CHO‐K1 cells expressing a recombinant melanocortin receptor (MC3R, MC4R and MC5R membranes are available from Perkin Elmer Life Sciences) Culture medium (see recipe
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