Rat Protein Red, IK ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
IK,Protein Red, Protein RER,IK factor,CSA2; RED; IK factor; RD element; chondrosarcoma-associated protein 2; cytokine IK; prer protein; IK cytokine, down-regulator of HLA II
Search name
Rat IK ELISA KIT ,Rat Protein Red ELISA KIT ,Rat Protein RER ELISA KIT ,Rat IK factor ELISA KIT ,Rat CSA2 ELISA KIT ,Rat RED ELISA KIT ,Rat IK factor ELISA KIT ,Rat RD element ELISA KIT ,Rat chondrosarcoma-associated protein 2 ELISA KIT ,Rat cytokine IK ELISA KIT ,Rat prer protein ELISA KIT ,Rat IK cytokine down-regulator of HLA II ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Protein Red concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Red. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein Red and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein Red, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein Red in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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