- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-22003r
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat ATP-dependent DNA helicase PIF1,Pif1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat ATP- dependent DNA helicase PIF1, PIF1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Rat PIF1 ELISA KIT ,Rat PIF1 5'-to-3' DNA helicase ELISA KIT ,Rat PIF1/RRM3 DNA helicase-like protein ELISA KIT ,Rat PIF1 5'-to-3' DNA helicase homolog ELISA KIT ,Rat DNA repair and recombination helicase PIF1 ELISA KIT ,Rat C15orf20 ELISA KIT ,Rat PIF ELISA KIT ,Rat petite integration frequency 1 ELISA KIT ,Rat ATP-dependent DNA helicase PIF1 ELISA KIT ,Rat PIF1/RRM3 DNA helicase-like protein ELISA KIT ,Rat PIF1/RRM3 DNA helicase-like protein ELISA KIT
Intended use
Rat PIF1 ELISA kit allows for the in vitro quantitative determination of Rat ATP- dependent DNA helicase PIF1, PIF1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat PIF1 ELISA kit has been pre-coated with an antibody specific to Rat PIF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat PIF1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat PIF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat PIF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Recombinant Yeast mtDNA Helicases Purification and Functional Assays
At least two DNA helicases are important for maintenance of the yeast Saccharomyces cerevisiae mitochondrial DNA (mtDNA). Pif1 protein is a 5′->3′ DNA helicase that affects the stability of mtDNA at higher temperatures but is not essential
，cGMP定量检测的变革。 cAMP和cGMP单克隆抗体成功开发 NewEast Biosciences（纽斯特）公司的科学家经过两年的研发，筛选了4万多个克隆，终于从中发现了高特异性，高亲和力的cAMP和cGMP单克隆抗体。并成功构建了世界上唯一的基于单抗的cAMP和cGMP ELISA检测试剂盒。这个cAMP（cGMP）单抗对非乙酰化的cAMP（cGMP）的特异性识别能力是其对cGMP（cAMP）、ATP（GTP）以及其他核苷类似物识别能力的108倍以上，这相比
Biochemical Assays for the Characterization of DNA Helicases
Helicases are ubiquitous enzymes that disrupt complementary strands of duplex nucleic acid in a reaction dependent on nucleoside-5′-triphosphate hydrolysis. Helicases are implicated in the metabolism of DNA structures that are generated
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