- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-20712r
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Peptide-N(4)-(N-acetyl-beta-glucosaminyl) asparagine am
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Peptide- N(4)- (N- acetyl- beta- glucosaminyl) asparagine amidase, NGLY1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NGLY1,CDG1V; PNG1; PNGase; hPNGase; peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; peptide:N-glycanase; N-glycanase 1
Search name
Rat NGLY1 ELISA KIT ,Rat CDG1V ELISA KIT ,Rat PNG1 ELISA KIT ,Rat PNGase ELISA KIT ,Rat hPNGase ELISA KIT ,Rat peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase ELISA KIT ,Rat peptide:N-glycanase ELISA KIT ,Rat N-glycanase 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Peptide- N(4)- (N- acetyl- beta- glucosaminyl) asparagine amidase, NGLY1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGLY1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NGLY1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NGLY1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NGLY1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody Detection
to the sequences of human and rat acetylcholine receptor subunits was immobilized on glass and silicon slides coated by a copolymer of N ,N -dimethylacrylamide, N -acryloyloxysuccinimide, and 3-(trimethoxysilyl) propyl methacrylate, copoly(DMA–NAS–MAPS
。因为Cys、Met、Trp或者其侧链很容易氧化。如果可能的话应尽量避免这些残基在序列中出现，或者做一些保守的替换。例如，用Ser代替Cys，Norleucine代替Met，Tyr、Phe或其他一些疏水性残基如Leu代替Trp。Lys可以用来代替Arg。 改善溶解性的多肽序列的设计方案1. 改变序列的N或C端对酸性肽（即在中性条件下多肽带负电荷），我们推荐多肽为这样的形式：Acetyl-peptide-COOH（多肽N端乙酰化，C端自由羧基），以使多肽尽可能多的带负电荷。对碱性肽（即在中性条件下多肽
Chemical and Enzymatic Release of Glycans from Glycoproteins
of one or more oligosaccharide (glycan) chains. Glycans may be attached to the peptide backbone through different types of linkage but they usually are subdivided into those attached to glycoproteins primarily through an amide linkage to asparagine
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