- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-20316r
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Hypoxanthine-guanine phosphoribosyltransferase,Hprt1 ELISA
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Hypoxanthine- guanine phosphoribosyltransferase, HPRT1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HPRT1,HGPRT; HPRT; HGPRTase; hypoxanthine-guanine phosphoribosyltransferase 1; hypoxanthine phosphoribosyltransferase 1, Hypoxanthine-guanine phosphoribosyltransferase
Search name
Rat HPRT1 ELISA KIT ,Rat HGPRT ELISA KIT ,Rat HPRT ELISA KIT ,Rat HGPRTase ELISA KIT ,Rat hypoxanthine-guanine phosphoribosyltransferase 1 ELISA KIT ,Rat hypoxanthine phosphoribosyltransferase 1 ELISA KIT ,Rat Hypoxanthine-guanine phosphoribosyltransferase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat hypoxanthine-guanine phosphoribosyltransferase,HGPRT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HGPRT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HGPRT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HGPRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HGPRT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes
Assays for in vivo mutation are used to identify genotoxic hazards and phenotypes prone to genomic instability and cancer. The hypoxanthine guanine phosphoribosyl transferase (Hprt ) gene and the phosphatidyl inositol glycan, class A (Pig
The T-cell cloning assay, which detects mutations in the gene for hypoxanthine-guanine phosphoribosyltransferase (HPRT ), is the most well-developed reporter system for studying specific locus mutation in human somatic cells. The assay
Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes
-throughput method for identifying mouse spleen lymphocytes having mutations in the endogenous X-linked hypoxanthine-guanine phosphoribosyltransferase (Hprt ) gene and the endogenous autosomal thymidine kinase (Tk ) gene. The selective expansion of mutant
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