Rat Uromodulin, Umod ELISA试剂盒

Rat Uromodulin, UMOD ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Uromodulin,Tamm-Horsfall urinary glycoprotein(THP), UMOD,ADMCKD2; FJHN; HNFJ; HNFJ1; MCKD2; THGP; THP; Tamm-Horsfall

glycoprotein; uromodulin (uromucoid, Tamm-Horsfall glycoprotein); uromucoid; uromodulin

Search name

Rat Uromodulin ELISA Kit, Rat UMOD ELISA Kit, Rat Tamm-Horsfall urinary glycoprotein ELISA Kit, Rat THP

ELISA Kit, Rat ADMCKD2 ELISA Kit, Rat FJHN ELISA Kit, Rat HNFJ ELISA Kit, Rat HNFJ1 ELISA Kit, Rat

MCKD2 ELISA Kit, Rat THGP ELISA Kit, Rat THP ELISA Kit, Rat Tamm-Horsfall glycoprotein ELISA Kit, Rat

uromucoid ELISA Kit,

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Uromodulin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uromodulin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Uromodulin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Uromodulin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Uromodulin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF Manual download