Rat Inhibitor of nuclear facto

Rat Inhibitor of nuclear factor kappa- B kinase subunit beta, Ikbkb ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Inhibitor of nuclear factor kappa-B kinase subunit beta,I-kappa-B-kinase beta,IKK-B,IKK-beta,IkBKB,I-kappa-B kinase 2,IKK2,Nuclear factor NF-kappa-B inhibitor kinase beta,NFKBIKB, IKBKB,IKKbeta,IKK-beta; IKK2; IKKB; IMD15; NFKBIKB; I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK-B; inhibitor of nuclear factor kappa-B kinase subunit beta; nuclear factor NF-kappa-B inhibitor kinase beta; inhibitor of kappa light polypeptide gene enhancer in B-cells kinase beta

Search name

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Intended use

Rat IKBKB ELISA KIT allows for the in vitro quantitative determination of rat inhibitor of nuclear factor kappa-b kinase subunit beta,IKBKB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to I-kappa-B-kinase beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for I-kappa-B-kinase beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain I-kappa-B-kinase beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of I-kappa-B-kinase beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.312-20.0 ng/ml

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