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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Neuromedin-U,Nmu
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Neuromedin U, NMU ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Neuromedin-U, prepro-NMU; neuromedin-U,Nmu
Search name
Rat NMU ELISA Kit, Rat Neuromedin-U ELISA Kit, Rat Neuromedin U ELISA Kit, Rat prepro-NMU ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Neuromedin-U concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuromedin-U. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neuromedin-U and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuromedin-U, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neuromedin-U in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验三句话读懂一篇 CNS:限时饮食 vs 热量限制,哪个减重效果更好?为何男性更易患癌且生存率更低?
into the peptide selectivity and activation of human neuromedin U receptors。 该研究首次报道了两类神经调节肽 U 受体亚型(Neuromedin U receptor 1/2, NMUR1/2)分别结合内源多肽激素即神经调节肽 U(Neuromedin U, NMU)和神经调节肽 S(Neuromedin S, NMS)以及 Gq 蛋白信号复合体的 4 个近原子分辨率结构,阐释了该家族成员独特的配体识别选择性和受体激活的分子机制,有助于增加对神经
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
l/well of 1:625 Biotinylated Goat anti-rat IgG prepared by adding 32 µl IgG (Calbiochem #60653) to 20 ml blocking buffer with the addition of 200 µl of 10% Tween. Return plate to the 4°C on the rotator for 6 hours.• APPLYING STREPTAVIDINAfter 6 hours
www.westang.com 上海西唐生物科技公司 021-55229872 分装 ELISA 试剂盒 货号 品名 方法
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