- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-05660r
- 2026年01月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat 6-phosphofructokinase type C,Pfkp
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat 6- phosphofructokinase type C, Pfkp ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PFKP,PFK-C; PFKF; 6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; Phosphofructokinase, platelet type; phosphofructo-1-kinase isozyme C; phosphofructokinase 1; phosphohexokinase; phosphofructokinase, platelet ,
,Phosphofructo-1-kinase isozyme C,Phosphofructokinase 1,
Search name
Rat PFKP ELISA KIT ,Rat PFK-C ELISA KIT ,Rat PFKF ELISA KIT ,Rat 6-phosphofructokinase type C ELISA KIT ,Rat 6-phosphofructokinase ELISA KIT ,Rat platelet type ELISA KIT ,Rat Phosphofructokinase ELISA KIT ,Rat platelet type ELISA KIT ,Rat phosphofructo-1-kinase isozyme C ELISA KIT ,Rat phosphofructokinase 1 ELISA KIT ,Rat phosphohexokinase ELISA KIT ,Rat phosphofructokinase platelet ELISA KIT , Rat Phosphofructo-1-kinase isozyme C ELISA KIT ,Rat Phosphofructokinase 1 ELISA KIT ,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat 6-phosphofructokinase type c,6-phosphofructokinase type C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to 6-phosphofructokinase type C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 6-phosphofructokinase type C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 6-phosphofructokinase type C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 6-phosphofructokinase type C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验1 fibers in immunofluorescence and with rat type 1 MHCs (myosin heavy chain)in Western blotting;the cell line was established in 1986 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse that had been immunized with purified myosin
对肝纤维化的发生、发展发挥重要作用呢？我们课题组的先前研究表明在肝纤维大鼠的肝组织中HMGB1的表达明显增强，但HMGB1是否通过激活HSC参与肝纤维化的发生发展目前尚不清楚，本研究拟通过HMGB1刺激HSC以观察ECM的合成情况来进一步探讨HMGB1与肝纤维化的关系。 1 材料与方法 1.1 主要试剂 HMGB1由本室制备保存；新生胎牛血清（FCS）为杭州四季青生物工程材料研究所产品；高糖型DMEM购自英国Gibco公司；大鼠PⅢP ，CⅣ双抗体夹心ELISA检测试剂盒购自
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
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