- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-05413r
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Growth/ differentiation factor 8,GDF8
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Growth/differentiation factor 8,GDF8 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MSTN,myostatin; GDF8; MSLHP,Growth/differentiation factor 8
Search name
Rat MSTN ELISA KIT,Rat myostatin ELISA KIT,Rat GDF8 ELISA KIT,Rat MSLHP ELISA KIT, Rat Growth/differentiation factor 8 ELISA KIT, Rat GDF-8 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat growth/differentiation factor 8,GDF-8 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF-8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GDF-8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GDF-8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GDF-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
CalbiochemReferenceWeskamp, G., and Otten, U. (1987). An enzyme-linked immunoassay for nerve growth factor (NGF): A tool for studying regulatory mechanisms involved in ngf production in brain and in peripheral tissues. Journal of Neurochemistry, 48 (6),1779-1786.
out at about 1-3x105 cells/ml;at high cell density(>2 x 106 cells/ml),a cytokine-independent subclone may grow out relatively quickly.CAVEAT:by favoring selective growth,suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent
/j.1365-2133.2010.09699.x BJD9699 [pii] (2010). 7. Fusenig, N.E., et al . Growth and differentiation characteristics of transformed keratinocytes from mouse and human skin in vitro and in vivo . J. Invest. Dermatol. 81, 168s-175s (1983
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