- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-05193r
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Fibronectin type III domain-containing protein 5,Fndc5 ELI
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Fibronectin type III domain- containing protein 5, FNDC5 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
FNDC5,FRCP2; irisin; fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2; fibronectin type III domain containing 5, Peroxisomal protein,PeP,
Search name
Rat FNDC5 ELISA KIT ,Rat FRCP2 ELISA KIT , Rat Peroxisomal protein ELISA KIT , Rat PeP ELISA KIT ,Rat irisin ELISA KIT ,Rat fibronectin type III domain-containing protein 5 ELISA KIT ,Rat fibronectin type III repeat-containing protein 2 ELISA KIT ,Rat fibronectin type III domain containing 5 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat fibronectin type iii domain-containing protein 5,FNDC5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to FNDC5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FNDC5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FNDC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FNDC5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验三句话读懂一篇 CNS：老年血液会让年轻个体未老先衰？不抽烟不喝酒可大幅延长预期寿命
Journal 杂志发表研究论文 Exerkine fibronectin type-III domain-containing protein 5/irisin-enriched extracellular vesicles delay vascular ageing by increasing SIRT6 stability。 该研究发现富含 FNDC5/irisin 的 EV 有助于运动诱导的血管老化保护，表明运动因子 FNDC5/irisin 可能是与衰老相关的血管合并症的潜在靶点。图 10
domain 428 490 ig-like c2-type domain. domain 518 589 ig-like c2-type domain. domain 599 608 hinge (potential). domain 601 607 gly/pro-rich. domain 608 709 fibronectin type-iii. domain 710 811 fibronectin type-iii. domain 812 912 fibronectin
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
strain SNS‐126 containing the two‐hybrid reporter and protein domains of interest (or control protein) fused to the bacteriophage repressor DNA‐binding domains ( protocol 2 ) 50 to 200 ng
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