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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Neuronal protein 3.1,P311
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat P311 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat P311 ELISA KIT ,Rat protein p311 ELISA KIT ,Rat C5orf13 ELISA KIT ,Rat D4S114 ELISA KIT ,Rat PRO1873 ELISA KIT ,Rat PTZ17 ELISA KIT ,Rat SEZ17 ELISA KIT ,Rat neuronal regeneration-related protein ELISA KIT ,Rat neuronal protein 3.1 ELISA KIT ,Rat neuronal regeneration related protein homolog ELISA KIT ,Rat neuronal regeneration related protein ELISA KIT
Intended use
Rat P311 ELISA kit is for the in vitro quantitative determination of Rat P311 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Rat P311 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for P311 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain P311, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of P311 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat P311 ELISA kit Range 78-5000pg/ml
P311 Related Products
> P311 ELISA KITS
Human Neuronal protein 3.1, P311 ELISA KIT
Mouse Neuronal protein 3.1, P311 ELISA KIT
Rat Neuronal protein 3.1, P311 ELISA KIT
> P311 Recombinant Protein
Human P311 Recombinant Protein
> P311 Antidodies
Anti P311 Antidody
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
The promoter activity of the rat Ca2+ /calmodulin-dependent protein kinase II gene was analyzed using the luciferase reporter gene in neuronal and non-neuronal cell lines. Neuronal cell type-specific promoter activity was found in the 5′-flanking
Measurement of Chloride Movement in Neuronal Preparations
to chloride channels. Similar chloride flux assays for primary neuronal cultures are also presented. Alternatively, the efflux of chloride from synaptoneurosomes and primary neuronal cultures can be studied using fluorescent dyes and photometry. Finally
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