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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Metallothionein-1,MT-1
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Metallothionein-1,MT-1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MT1A,MT1;MT, MT1S; MTC; MT-1A; MT-IA; metallothionein 1A ,metallothionein 1S; metallothionein-IA; metallothionein 1A, metallothionein
Search name
Rat MT1A ELISA KIT , Rat MT ELISA KIT ,Rat MT1 ELISA KIT , Rat metallothionein ELISA KIT Rat MT1S ELISA KIT ,Rat MTC ELISA KIT ,Rat MT-1A ELISA KIT ,Rat MT-IA ELISA KIT ,Rat metallothionein 1A ELISA KIT ,Rat metallothionein 1S ELISA KIT ,Rat metallothionein-IA ELISA KIT ,Rat metallothionein 1A ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat metallothionein-1,MT-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MT-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MT-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MT-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人金属硫蛋白 ( MT )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 MT( Metallothionein ) 单抗包被于酶标板上,标准品和样品中的 MT与单抗结合,加入生物
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠金属硫蛋白 (MT)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 MT( Metallothionein ) 单抗包被于酶标板上,标准品和样品中的 MT 与单抗结合,加入生物素化的抗大鼠 MT ,形成免疫
Lipsosomes: 1:1 molar ratio of 1,2‐dioleoyl‐3‐trimethylammonium‐propane (DOTAP)/cholesterol (see Ma et al., , b ) Fluorescent reporter construct: e.g., FRET‐MT
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