Rat Apoptosis- inducing factor 1, mitochondrial, Aifm1 ELISA KIT
Product Name:Rat Apoptosis- inducing factor 1, mitochondrial, Aifm1 ELISA KIT
Packing:96T
Catalog No.:ELI-03459r
Gene Name:Aifm1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Rat Apoptosis- inducing factor 1 mitochondrial
Alternative Name:Aifm1,Rat Apoptosis- inducing factor 1 mitochondrial
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat Apoptosis- inducing factor 1, mitochondrial, Aifm1 ELISA KIT allows for the in vitro quantitative determination ofRat Apoptosis- inducing factor 1 mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rat Apoptosis- inducing factor 1, mitochondrial, Aifm1 ELISA KIT has been pre-coated with an Aifm1 antibody specific to Rat Apoptosis- inducing factor 1 mitochondrial .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat Apoptosis- inducing factor 1 mitochondrial and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Apoptosis- inducing factor 1 mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat Apoptosis- inducing factor 1 mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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