- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-03024r
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Tartrate-resistant acid phosphatase type 5,TRAP
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Tartrate- resistant acid phosphatase type 5, TRAP ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ACP5,TRAP; HPAP; SPENCDI; TrATPase; rat purple acid phosphatase; tartrate-resistant acid ATPase; acid phosphatase 5 tartrate resistant, Tartrate-resistant acid phosphatase type 5,TR-AP,Type 5 acid phosphatase, Uteroferrin,UF,
Search name
Rat ACP5 ELISA KIT ,Rat TRAP ELISA KIT ,Rat HPAP ELISA KIT ,Rat SPENCDI ELISA KIT ,Rat TrATPase ELISA KIT ,Rat rat purple acid phosphatase ELISA KIT ,Rat tartrate-resistant acid ATPase ELISA KIT ,Rat acid phosphatase 5 tartrate resistant ELISA KIT ,Rat Tartrate-resistant acid phosphatase type 5 ELISA KIT ,Rat TR-AP ELISA KIT ,Rat Type 5 acid phosphatase ELISA KIT,Rat Uteroferrin ELISA KIT, ,Rat UF ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat tartrate-resistant acid phosphatase type 5,TR-AP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TR-AP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TR-AP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TR-AP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TR-AP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Analysis of Osteoclast Function in Mouse Calvarial Cultures
can produce pits (8 ), and two distinct morphologies of multinucleate tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells have been described with differing resorptive capacities (9 ). Like the osteoclast, the foreign body giant cell
Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development
for calcitonin receptor and tartrate-resistant acid phosphatase (TRAP), which are specific markers for this lineage, and then they fuse with each other and form fully functional multinucleated large cells ( 1 , 2 ) (Fig. 1 ). TRAP-positive osteoclasts
Osteoclast Formation in the Mouse Coculture Assay
, osteoclasts can be identified by tartrate-resistant acid phosphatase (TRAP) staining, and, when the cultures are performed on dentine slices, resorption activity can be measured as well. Even though today it is possible to generate osteoclasts from bone marrow
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