Rat Renin, REN ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 3.4.23.15,Renin,Angiotensinogenase, REN,REN1,REN-1,renin precursor, renal; HNFJ2; angiotensin-forming enzyme, Kidney renin
Search name
Rat Class 3.4.23.15 ELISA KIT ,Rat Renin ELISA KIT ,Rat Angiotensinogenase ELISA KIT ,Rat REN ELISA KIT ,Rat REN1 ELISA KIT ,Rat REN-1 ELISA KIT ,Rat renin precursor ELISA KIT ,Rat renal ELISA KIT ,Rat HNFJ2 ELISA KIT ,Rat angiotensin-forming enzyme ELISA KIT ,Rat Kidney rennin ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat renin,Renin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Renin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Renin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Renin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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