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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Prolactin,Prl
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Prolactin, PRL ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Prolactin(PRL),; decidual prolactin
Search name
Rat PRL ELISA KIT ,Rat Prolactin ELISA KIT ,Rat decidual prolactin ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat prolactin,PRL concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PRL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PRL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验腺垂体分泌的一种蛋白质激素,由199个氨基酸残基所组成。其对乳腺与泌乳的作用主要为促进乳腺发育生长,引起并维持泌乳。女性青春期乳腺发育主要由于雌激素刺激,孕激素、生长素等也起协同作用。妊娠期,催乳素、人绒毛膜生长素、孕激素、雌激素使乳腺组织进一步发育,由于血液中雌激素、孕激素浓度过高,与催乳素竞争乳腺细胞受体,催乳素失去效力。分娩后,血中孕激素、雌激素浓度降低,催乳素发挥启动和维持泌乳作用。胎儿垂体能分泌、贮存与释放催乳素。到分娩前几周,其在血中浓度达高峰。羊水中其浓度
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠催乳素 (PRL)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 PRL 单抗包被于酶标板上,标准品和样品中的 PRL与单抗结合,加入生物素化的抗小鼠 PRL ,形成免疫
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠催乳素 (PRL)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 PRL 单抗包被于酶标板上,标准品和样品中的 PRL 与单抗结合,加入生物素化的抗大鼠 PRL ,形成免疫复合物连接在板上,辣根
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