Rat Aminolevulinate Delta Dehy

Rat Aminolevulinate Delta Dehydratase, ALADH ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

4.2.1.24,Delta-aminolevulinic acid dehydratase,ALADH,Porphobilinogen synthase,; ALAD,LV,ALADH; PBGS; aminolevulinate, delta-, dehydratase; delta-aminolevulinic acid dehydratase; porphobilinogen synthase; aminolevulinate dehydratase

Search name

Rat Class 4.2.1.24 ELISA KIT ,Rat Delta-aminolevulinic acid dehydratase ELISA KIT ,Rat ALADH ELISA KIT ,Rat Porphobilinogen synthase ELISA KIT ,Rat ALAD ELISA KIT ,Rat LV ELISA KIT ,Rat ALADH ELISA KIT ,Rat PBGS ELISA KIT ,Rat aminolevulinate ELISA KIT ,Rat delta- ELISA KIT ,Rat  dehydratase ELISA KIT ,Rat delta-aminolevulinic acid dehydratase ELISA KIT ,Rat porphobilinogen synthase ELISA KIT ,Rat aminolevulinate dehydratase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat delta-aminolevulinic acid dehydratase,ALADH concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALADH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ALADH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ALADH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ALADH in the samples is then determined by comparing the O.D. of the samples to the standard curve.